Hematoxylin and Eosin Stain for Glycol Methacrylate Sections
Technovit Glycol Methacrylate: Technical Information and Help
From Heraeus Kulzer
- Hematoxylin and Eosin stain is a good general stain for many
types of tissue.
- Hematoxylin (C.I. 75290) - 6 g
- Sodium Iodate - 0.6 g
- Aluminum Sulfate - 52.8 g
- Distilled Water - 690 ml
- Ethylene Glycol - 250 ml
- Glacial Acetic Acid - 60 ml
- Eosin Y (alcoholic C.I. 45380)- 0.5 g
- Ethanol 96% - 100 ml
- Glacial Acetic Acid - 2 drops
- 4 ml of 25% HCl.
- 100 ml of 70% Ethanol.
- Stain in Gill's hematoxylin for 15 minutes.
- Wash in tap water for 10 minutes.
- Decolorize plastic, if necessary, with acid alcohol followed
by tap water rinse.
- Rinse in distilled water.
- Scott's Tap Water for 1 - 2 minutes.
- Two changes of distilled water.
- Counterstain with Eosin for 2 - 5 minutes.
- Dehydrate using 96% and 100% ethanol.
- Clear in xylene and coverslip.
Nuclei - blue
Basophilic cytoplasm - blue
Acidophilic cytoplasm - pink
Muscle tissue - pink
Connective tissue - pink
Gerrits, P. O. and Smid, L., "Staining Procedures for Tissues
Embedded in 2-Hydroxyethyl Methacrylate", Heraeus Kulzer.