Oil Red O Stain for Glycol Methacrylate Sections Staining Procedures For Plastic Embedded Tissue
Bob Schoonhoven, Laboratory of Molecular Carcinogenesis and
Mutagenesis, Dept. of Environmental Sciences and Engineering,
University of North Carolina, CB#7400, Chapel Hill, NC 27599
INDICATIONS:
Demonstrate lipids SOLUTIONS
60% aqueous triethyl phosphate
| 0.5% Oil Red O solution |
|
0.5g |
|
Oil Red O (CI 26125 |
|
100.0ml |
|
60% aqueous triethyl phosphate
Filter before use |
|
|
|
|
| Celestin Blue |
|
0.5g |
|
celestin blue B |
|
100 ml |
|
5% aqueous ferric ammonium sulfate
Boil gently 2-3 minutes; cool to room temperature; filter; and add 12
ml glycerol
Filter before use |
PROCEDURE:
Rinse briefly in 60% triethyl phosphate
Stain 5-20 minutes in Oil Red O solution
Rinse 1-2 seconds in 60% triethyl phosphate
Rinse well in distilled water
Counterstain in Celestin Blue 15 minutes
Rinse well in distilled water
Mount in glycerin jelly or other water-soluble mount RESULTS:
lipids: red-orange
nuclei: blue CITATION:
Feldman, A.T. and Dapson, R.W., "Relative Effectiveness
of Various Solvents for Oil Red O," Medical Laboratory
Technology, Vol. 31:335-341, 1974.
Disclaimer:
Energy Beam Sciences manufactures the JB-4
and JB-4A microtomes for sectioning plastic-embedded tissue,
and sells GMA kits. Hematoxylin and Eosin stain
is a good general stain for many types of tissue. |
