Prussian Blue Stain for Glycol Methacrylate Sections

Technovit Glycol Methacrylate: Technical Information and Help From Heraeus Kulzer.

Prussian Blue Reaction according to Perls

Procedure:

  1. Stain in Potassium Ferrocyanide solution at 60C for 15 minutes. Filter solution after heating and before use.
  2. Wash in distilled water.
  3. Stain in Safranin O for 2 - 5 minutes.
  4. Wash in 1% acetic acid.
  5. Dehydrate in 96% alcohol two times then 100% alcohol.
  6. Clear in xylene and mount.

Results:

Nuclei - red
Hemosiderin - blue/green

Solutions:

A. Potassium Ferrocyanide Solution
  • Potassium Ferrocyanide - 1 gm
  • Distilled Water - 50 ml
  • 2% Hydrochloric Acid - 50 ml
B. Safranin O
  • Safranin O (C.I.#50240) - 0.2 gm
  • 1% Acetic Acid - 100 ml

Citation:

Gerrits, P. O. and Smid, L., "Staining Procedures for Tissues Embedded in 2-Hydroxyethyl Methacrylate", Heraeus Kulzer.


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