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Material Safety Data Sheets

 

Preserve Fixative
POL Metal Polish
Technovit® 2060 liquid
Technovit® 2060 powder
Technovit® 3040 liquid
Technovit® 3040 powder
Technovit® 7100 liquid
Technovit® 7100 hardener 1
Technovit® 7100 hardener 2
Technovit® 8100 liquid
Technovit® 8100 hardener 1
Technovit® 8100 hardener 2
Technovit® 9100 liquid
Technovit® 9100 hardener 1
Technovit® 9100 hardener 2
Technovit® 9100 powder
Technovit® 9100 regulator

NOTE: GMA resin-based products (Technovit® 7100, 8100) should not be used when epoxy removal from specimens is required. There is no known method of GMA epoxy removal (including methoxide) that does not effectively render specimens unusable. Instead, MMA resin-based products such as Technovit® 9100 should be selected.

 

Applications, Techniques, and Protocols

 

Microwave Fixation

Microwave Stimulated Fixation With Preserve

Microwave Applications, Techniques and Procedures

The Microwave Companion for histology

  • Protocols
  • Special Stains
  • Fixation
  • Tissue Processing
  • Heath
  • Tips & Tricks
  • Safety
  • Accreditation
  • Regulation
  • Testing
  • FAQs

Special Stains Using the Microwave
Joyce Moore of Jefferson Regional Medical Center, Pine Bluff AR

Microwave Processing Techniques for Microscopy
Energy Beam Sciences, Inc.

References for Microwave Polymerization of EM Resins

Microwave Antigen Retrieval Technique
Shi, Shan-Rong et al., "Antigen retrieval technique: A novel approach to immunohistochemistry on routinely processed tissue sections," , in Gu, J, ed. Analytical Morphology: Theory, Applications and Protocols, BioTechniques Books, div. of Eaton Publishing, 1997.

Microwave Irradiation of Cryostat Sections Accelerates and Improves Nitric Oxide Synthase Staining,
Michele Forte and Jiang Gu, Cell Vision, Vol. 1, No. 1, 1994. posted with permission.

Working Procedures in Microwave Histology,
Vincent R. Klump, Jr., HT (ASCP), Histology Services, East Haven, CT

Microwave Tissue Processing
From Donna Willis, Harris Methodist Hospital, Ft. Worth, Texas

Microwave Oven Quality Control: Measuring Output
From Donna Willis, Harris Methodist Hospital, Ft. Worth, Texas

Microwave Biopsy Histoprocessing Procedure III
From Denise Hardy, ARUP Laboratories, Salt Lake City, UT

Accumate ô RDO Rapid bone Decalcification Procedure

Immunolabeling

Immunogold Labeling with BioSite Colloidal Gold Reagents
BioSite Cationic Colloidal Gold
Immunogold Labelling in Scanning Electron Microscopy

General Histology

Eliminate Xylene from Your Lab

Plastic Embedded Tissue

Staining Procedures For Plastic Embedded Tissue
Verified at the Applications Laboratory of the Biomedical Division, Sorvall Microtomes. 

Acid Orcein -- Azure-Eosin
Aldehyde Fuchsin, Gomori's
Azan, Modified Heidenhain's
Azure-Eosin, Modified Nocht's
Amyloid Congo Red (Alkaline)
Giemsa Hematoxylins
Methyl Green -- Pyronin For Plasma Cells
Methyline Blue -- Basic Fuchsin, Lee's
Schiff Stains
Silver Stains
Van Gieson Stain

Oil Red O Stain
Bob Schoonhoven, University of North Carolina

Alkaline Phosphatase for Osteoblasts
Patsy Ruegg, Metabolic Bone Disease Laboratory of the University of Colorado Health Sciences Center

Acid Phosphatase

Glycol Methacrylate: Embedding and Staining

Technovit® 7100 and 8100 Application Information from Heraeus Kulzer Embedding Protocols

For Normal, Soft Tissue
For Immunohistochemistry
For Materials Samples

Sectioning and Mounting Protocols
Preparing Silanized Slides

Staining Protocols
Protocols for Lymphoid Tissue
Hematoxylin and Eosin
Toluidene Blue
Prussian Blue
Periodic Acid Schiff
Giemsa Stain
Acid Phosphatase (Barka & Anderson)
Alkaline Phosphatase (Burstone)

Methyl Methacrylate: Embedding and Staining

Technovit® 9100 Application Information from Heraeus Kulzer

Using Components
Tissue Preparation
Microtomy
Staining
Recipes & Reagents

Spurr Epoxy Sections

Diagnostic Electron Microscopy on Reembedded ("Popped Off") Areas of Large Spurr Epoxy Sections
P. Anthony di Sant'Agnese and Karen L. De Mesy-Jensen

Large Block Embedding and "Pop-Off" Technique for Immunoelectron Microscopy
Karen L. De Mesy-Jensen and by P. Anthony di Sant'Agnese

SEM Sample Preparation

Plasma Chemistry: Applications of Barrel Reactors
Application Note from Energy Beam Sciences

Plasma Chemistry Applied to Electron Microscopy (EM) Preparation Procedures
Application Note from Energy Beam Sciences

Carbon Coating Thickness
Dr. Norman Charnley, Department of Earth Sciences, University of Oxford

Microtomy Techniques

Vibratome® Operation
Application Note from Energy Beam Sciences

 


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